Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen.
نویسنده
چکیده
Manual and automated methods for the determination of blood glucose have been devised using an oxidase/peroxidase system, with dl adrenaline, a non-carcinogen, as oxygen acceptor. The manual technique employs a stable single solution protein precipitant and the other reagents used are also stable. The automated methods are operated at 40/hr sample speed and washover between samples, over a very wide concentration range, is negligible.
منابع مشابه
Spectrophotometric or fluorometric methods with glucose oxidase (GOD)–peroxidase (POD)–chromogen(s) systems
oxidase (GOD)–peroxidase (POD)–chromogen(s) systems are well known as useful tools for determination of glucose. Indicator reactions in these methods are based on oxidative formation of a colored or fluorescent substance from a chromogen(s) with H2O2, generated through GODcatalyzed oxidation of glucose, in the presence of POD. These color reactions are well-characterized chemical processes. How...
متن کاملDetermination of glucose in biologic fluids with an automated enzymatic procedure.
An automated procedure for the enzymatic determination of “true” glucose with the glucose oxidase-peroxidase system is described. A threefold increase in sensitivity was obtained through meaurement of the stable, pink chromogen obtained at high acid concentration. Good reproducibility and stoichiometry was obtained over the range of 50-400 mg./l00 ml. at a sampling rate of 40/hr. The normal ran...
متن کاملA Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots
Glucose, as the major energy source in cellular metabolism, plays an important role in the natural growth of cells. Herein, a simple, rapid and low-cost method for the glucose determination by utilizing glucose oxidase and CdTe/thioglycolic acid (TGA) quantum dots (QDs) on a thin layer chromatography (TLC) plate has been described. The detection was based on the combination of the glucose enzym...
متن کاملNew chromogen for assay of glucose in serum.
We describe a colorimetric assay for glucose determination in human serum, with use of the chromogen 2-amino-4-hydroxybenzenesulfonic acid (AHBS), glucose oxidase, and peroxidase. With this assay, glucose concentrations less than or equal to 27.8 mmol/L can be measured in serum, with a sample/reagent volume ratio as low as 0.0025. The chromogen itself is easily soluble in water and does not req...
متن کاملEvaluation of Fasting Blood Sugar via Salivary Glucose in Type 2 Diabetes Mellitus
Objective: Diabetes mellitus (DM) as an ongoing metabolic disorder worldwide is a major public health concern. Diagnosis of DM is depended on clinical symptoms and laboratory findings. Fasting blood glucose (FBG) monitoring is currently the most common diagnostic method, which is an invasive and stressful procedure. Most recently, the use of non-invasive, convenient methods like fasting salivar...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 22 2 شماره
صفحات -
تاریخ انتشار 1969